[HTML][HTML] AT1 receptor mutant lacking heterotrimeric G protein coupling activates the Src-Ras-ERK pathway without nuclear translocation of ERKs
K Seta, M Nanamori, JG Modrall, RR Neubig… - Journal of Biological …, 2002 - ASBMB
Angiotensin II (Ang II) type 1 receptors (AT1Rs) activate tyrosine kinases, including Src.
Whether or not tyrosine kinase activation by AT1R occurs independently of heterotrimeric G
protein coupling and, if so, the cellular function of such a mechanism are unknown. To
address these questions, we used an AT1aR intracellular second loop mutant, which lacks
heterotrimeric G protein coupling (AT1a-i2m). Surprisingly, Ang II-induced Src activation was
preserved in AT1a-i2m, which was not attenuated by inhibiting protein kinase C and Ca 2+ …
Whether or not tyrosine kinase activation by AT1R occurs independently of heterotrimeric G
protein coupling and, if so, the cellular function of such a mechanism are unknown. To
address these questions, we used an AT1aR intracellular second loop mutant, which lacks
heterotrimeric G protein coupling (AT1a-i2m). Surprisingly, Ang II-induced Src activation was
preserved in AT1a-i2m, which was not attenuated by inhibiting protein kinase C and Ca 2+ …