[HTML][HTML] A differentiation-dependent splice variant of myosin light chain kinase, MLCK1, regulates epithelial tight junction permeability
DR Clayburgh, S Rosen, ED Witkowski, F Wang… - Journal of Biological …, 2004 - ASBMB
Activation of Na+-nutrient cotransport leads to increased tight junction permeability in
intestinal absorptive (villus) enterocytes. This regulation requires myosin II regulatory light
chain (MLC) phosphorylation mediated by MLC kinase (MLCK). We examined the
spatiotemporal segregation of MLCK isoform function and expression along the crypt-villus
axis and found that long MLCK, which is expressed as two alternatively spliced isoforms,
accounts for 97±4% of MLC kinase activity in interphase intestinal epithelial cells …
intestinal absorptive (villus) enterocytes. This regulation requires myosin II regulatory light
chain (MLC) phosphorylation mediated by MLC kinase (MLCK). We examined the
spatiotemporal segregation of MLCK isoform function and expression along the crypt-villus
axis and found that long MLCK, which is expressed as two alternatively spliced isoforms,
accounts for 97±4% of MLC kinase activity in interphase intestinal epithelial cells …