We isolated and identified a stress protein that is up-regulated in response to hypoxia in
primary-cultured glial cells. Protein-disulfide isomerase (PDI) was up-regulated not only by
hypoxia in glia in vitro, but also by transient forebrain ischemia in rats in vivo. To determine
whether newly synthesized PDI is involved in tolerance to ischemic stress, we carried out
two procedures to induce PDI gene expression in human neuroblastoma SK-N-MC cells, as
well as intrahippocampal injection following electroporation of an expression vector capable …