Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS
T Sasazuki, T Okazaki, K Tada… - Molecular …, 2004 - Elsevier
T Sasazuki, T Okazaki, K Tada, S Sakon-Komazawa, M Katano, M Tanaka, H Yagita…
Molecular immunology, 2004•ElsevierWe recently showed that TNF induces accumulation of reactive oxygen species (ROS) that
mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2-and TRAF5-
double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation
of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs
before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant
enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in …
mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2-and TRAF5-
double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation
of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs
before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant
enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in …
We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS.
Elsevier