Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity

RE Weir, AR Morgan, WJ Britton, CR Butlin… - Journal of …, 1994 - Elsevier
A whole blood assay is described to measure T cell mediated immune responses to leprosy
and provide an alternative to the conventional lymphocyte transformation test. Optimal
conditions were defined for the whole blood assay, and interferon-γ measurement was
found to be a more sensitive way of measuring responses than tritiated thymidine
incorporation. The assay was shown to be useful for investigating responses to a range of
leprosy antigens. A whole blood assay has the advantages of being quick, simple and …