[HTML][HTML] Delivery of Macromolecules into Cytosol Using Liposomes Containing Hemolysin from Listeria monocytogenes (∗)
KD Lee, YK Oh, DA Portnoy, JA Swanson - Journal of Biological Chemistry, 1996 - ASBMB
The cytosolic space of cells is an important but relatively inaccessible target for the delivery
of therapeutic macromolecules. Here we describe the efficient delivery of macromolecules
into the cytosolic space of macrophages from liposomes that contain listeriolysin O (LLO),
the hemolytic protein of Listeria monocytogenes that normally mediates bacterial passage
from phagosomes into cytosol. LLO was purified and encapsulated inside pH-sensitive
liposomes, along with other molecules to be delivered. When internalized by bone marrow …
of therapeutic macromolecules. Here we describe the efficient delivery of macromolecules
into the cytosolic space of macrophages from liposomes that contain listeriolysin O (LLO),
the hemolytic protein of Listeria monocytogenes that normally mediates bacterial passage
from phagosomes into cytosol. LLO was purified and encapsulated inside pH-sensitive
liposomes, along with other molecules to be delivered. When internalized by bone marrow …