Differential binding of peptides substituted at putative C-terminal anchor residues to HLA-DQ8 and DQ9 differing only at β57
M Oiso, T Nishi, T Ishikawa, Y Nishimura… - Human immunology, 1997 - Elsevier
HLA-DQ8 (DQA1* 0302-DQB1* 0302: DQβ57Ala) and DQ9 (DQA1* 0302-DQB1* 0303:
DQβ57Asp) differ only at β57, at which polymorphism reportedly confers distinct
susceptibility to insulindependent diabetes mellitus (IDDM). To identify the differential
peptide binding affected by β57, we determined DQ9-binding peptides by affinity-based
selection of a phage random peptide library using the biotinylated DQ9 complex.
Nonconservative single-residue substitution of a high-affinity DQ8and DQ9-binding peptide …
DQβ57Asp) differ only at β57, at which polymorphism reportedly confers distinct
susceptibility to insulindependent diabetes mellitus (IDDM). To identify the differential
peptide binding affected by β57, we determined DQ9-binding peptides by affinity-based
selection of a phage random peptide library using the biotinylated DQ9 complex.
Nonconservative single-residue substitution of a high-affinity DQ8and DQ9-binding peptide …