Biglycan is a member of the small leucine-rich proteoglycan family. Its core protein
comprises two chondroitin/dermatan sulfate attachment sites on serine 42 and serine 47,
respectively, which are the fifth and tenth amino acid residues, respectively, after removal of
the prepro peptide. Because the regulation of glycosaminoglycan chain assembly is not fully
understood and because of the in vivo existence of monoglycanated biglycan, mutant core
proteins were stably expressed in human 293 and Chinese hamster ovary cells in which i) …