[HTML][HTML] Functional differences in Kv1. 5 currents expressed in mammalian cell lines are due to the presence of endogenous Kvβ2. 1 subunits

VN Uebele, SK England, A Chaudhary… - Journal of Biological …, 1996 - ASBMB
The voltage-sensitive currents observed following hKv1. 5 α subunit expression in HEK 293
and mouse L-cells differ in the kinetics and voltage dependence of activation and slow
inactivation. Molecular cloning, immunopurification, and Western blot analysis demonstrated
that an endogenous L-cell Kvβ2. 1 subunit assembled with transfected hKv1. 5 protein. In
contrast, both mRNA and protein analysis failed to detect a β subunit in the HEK 293 cells,
suggesting that functional differences observed between these two systems are due to …