Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene

WS Chen, PZ Xu, K Gottlob, ML Chen… - Genes & …, 2001 - genesdev.cshlp.org
WS Chen, PZ Xu, K Gottlob, ML Chen, K Sokol, T Shiyanova, I Roninson, W Weng, R Suzuki
Genes & development, 2001genesdev.cshlp.org
The serine/threonine kinase Akt has been implicated in the control of cell survival and
metabolism. Here we report the disruption of the most ubiquitously expressed member of the
akt family of genes, akt1, in the mouse. Akt1−/− mice are viable but smaller when compared
to wild-type littermates. In addition, the life span of Akt1−/− mice, upon exposure to genotoxic
stress, is shorter. However, Akt1−/− mice do not display a diabetic phenotype. Increased
spontaneous apoptosis in testes, and attenuation of spermatogenesis is observed in Akt1 …
The serine/threonine kinase Akt has been implicated in the control of cell survival and metabolism. Here we report the disruption of the most ubiquitously expressed member of theakt family of genes, akt1, in the mouse. Akt1−/− mice are viable but smaller when compared to wild-type littermates. In addition, the life span of Akt1−/− mice, upon exposure to genotoxic stress, is shorter. However, Akt1−/− mice do not display a diabetic phenotype. Increased spontaneous apoptosis in testes, and attenuation of spermatogenesis is observed in Akt1−/− male mice. Increased spontaneous apoptosis is also observed in the thymi of Akt1−/− mice, and Akt1−/− thymocytes are more sensitive to apoptosis induced by γ-irradiation and dexamethasone. Finally, Akt1−/− mouse embryo fibroblasts (MEFs) are more susceptible to apoptosis induced by TNF, anti-Fas, UV irradiation, and serum withdrawal.
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