[HTML][HTML] Molecular determinants of Na+ channel function in the extracellular domain of the β1 subunit
KA McCormick, LL Isom, D Ragsdale, D Smith… - Journal of Biological …, 1998 - ASBMB
The rat brain voltage-gated Na+ channel is composed of three glycoprotein subunits: the
pore-forming α subunit and two auxiliary subunits, β1 and β2, which contain immunoglobulin
(Ig)-like folds in their extracellular domains. When expressed in Xenopus oocytes, β1
modulates the gating properties of the channel-forming type IIA α subunit, resulting in an
acceleration of inactivation. We have used a combination of deletion, alanine-scanning, site-
directed, and chimeric mutagenesis strategies to examine the importance of different …
pore-forming α subunit and two auxiliary subunits, β1 and β2, which contain immunoglobulin
(Ig)-like folds in their extracellular domains. When expressed in Xenopus oocytes, β1
modulates the gating properties of the channel-forming type IIA α subunit, resulting in an
acceleration of inactivation. We have used a combination of deletion, alanine-scanning, site-
directed, and chimeric mutagenesis strategies to examine the importance of different …