Recently, several studies have reported oligomerization of G protein-coupled receptors,
although the functional implications of this phenomenon are still unclear. Using fluorescence
resonance energy transfer (FRET) and coimmunoprecipitation (COIP), we previously
reported that the human thyrotropin (TSH) receptor tagged with green fluorescent protein
(TSHR GFP) and expressed in a heterologous system was present as oligomeric complexes
on the cell surface. Here, we have extended this biophysical and biochemical approach to …