Interaction of aggregated catecholamines and nucleotides with intragranular proteins
KH Berneis, U Goetz, M Da Prada… - … Schmiedeberg's archives of …, 1973 - Springer
KH Berneis, U Goetz, M Da Prada, A Pletscher
Naunyn-Schmiedeberg's archives of pharmacology, 1973•SpringerAnalytical ultracentrifugation at different sets of conditions in the temperature range between
10 and 34° C shows that mixtures of l-noradrenaline and adenosine-5′-triphosphate in the
presence of Ca 2+ always sediment jointly, ie with only one single schlieren peak together
with intragranular expanded proteins obtained from bovine adrenal medulla. In contrast, two
schlieren peaks are obtained on addition of a globular protein, such as serum albumin, to l-
noradrenaline-Ca 2-adenosine-5′-triphosphate mixtures. It is concluded that aggregates of …
10 and 34° C shows that mixtures of l-noradrenaline and adenosine-5′-triphosphate in the
presence of Ca 2+ always sediment jointly, ie with only one single schlieren peak together
with intragranular expanded proteins obtained from bovine adrenal medulla. In contrast, two
schlieren peaks are obtained on addition of a globular protein, such as serum albumin, to l-
noradrenaline-Ca 2-adenosine-5′-triphosphate mixtures. It is concluded that aggregates of …
Summary
Analytical ultracentrifugation at different sets of conditions in the temperature range between 10 and 34° C shows that mixtures of l-noradrenaline and adenosine-5′-triphosphate in the presence of Ca2+ always sediment jointly, i.e. with only one single schlieren peak together with intragranular expanded proteins obtained from bovine adrenal medulla. In contrast, two schlieren peaks are obtained on addition of a globular protein, such as serum albumin, to l-noradrenaline-Ca2-adenosine-5′-triphosphate mixtures. It is concluded that aggregates of l-noradrenaline and adenosine-5′-triphosphate interact with the expanded proteins present in the catecholamine storage organelles.
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