Fluorescence studies of nucleotide interactions with bovine adrenal chromogranin A
SH Yoo, JP Albanesi, DM Jameson - Biochimica et Biophysica Acta (BBA) …, 1990 - Elsevier
The binding of the fluorescent probe bis-ANS to chromogranin A, the major protein of
adrenal chromaffin vesicles, caused a marked enhancement and blue shift in the
fluorescence emission spectrum. The emission maximum shifted from 515 nm to 480 nm
and the yield increased approx. 75-fold upon addition of 10 μM chromogranin A to 1 μM bis-
ANS. Adenine nucleotides had clear effects on the bis-ANS fluorescence signal, while other
nucleotides such as GTP, UTP and CTP had no discernible effect. Specifically, ATP caused …
adrenal chromaffin vesicles, caused a marked enhancement and blue shift in the
fluorescence emission spectrum. The emission maximum shifted from 515 nm to 480 nm
and the yield increased approx. 75-fold upon addition of 10 μM chromogranin A to 1 μM bis-
ANS. Adenine nucleotides had clear effects on the bis-ANS fluorescence signal, while other
nucleotides such as GTP, UTP and CTP had no discernible effect. Specifically, ATP caused …