Thiazole orange: a new dye for reticulocyte analysis
LG Lee, CH Chen, LA Chiu - Cytometry: The Journal of the …, 1986 - Wiley Online Library
LG Lee, CH Chen, LA Chiu
Cytometry: The Journal of the International Society for Analytical …, 1986•Wiley Online LibraryThe purpose of this study was to find a 488‐nm excitable fluorescent dye for reticulocyte
analysis by the use of fluorescence activated cell cytometry. The chemical structure of
thioflavin T, a dye used for reticulocyte analysis with 457‐nm excitation, was used as a
model. Several dyes were synthesized and evaluated by quantum yield determination,
fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we
have named “thiazole orange”; analysis of several blood samples with thiazole orange gave …
analysis by the use of fluorescence activated cell cytometry. The chemical structure of
thioflavin T, a dye used for reticulocyte analysis with 457‐nm excitation, was used as a
model. Several dyes were synthesized and evaluated by quantum yield determination,
fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we
have named “thiazole orange”; analysis of several blood samples with thiazole orange gave …
Abstract
The purpose of this study was to find a 488‐nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457‐nm excitation, was used as a model. Several dyes were synthesized and evaluated by quantum yield determination, fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we have named “thiazole orange”; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage.
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