Rate of production of plasma and very-low-density lipoprotein (VLDL) apolipoprotein C-III is strongly related to the concentration and level of production of VLDL …

JS Cohn, BW Patterson, KD Uffelman… - The Journal of …, 2004 - academic.oup.com
JS Cohn, BW Patterson, KD Uffelman, J Davignon, G Steiner
The Journal of Clinical Endocrinology & Metabolism, 2004academic.oup.com
Overweight individuals with reduced insulin sensitivity often have mild to moderate
hypertriglyceridemia. To investigate the role of apolipoprotein (apo) C-III metabolism in the
etiology of hypertriglyceridemia in these individuals, we investigated 10 male subjects with
different body weights (body mass index, 24–34 kg/m2) and insulin sensitivity (homeostasis
model assessment, 4.7–35.0). Total plasma and very-low-density lipoprotein (VLDL) apoC-
III kinetics, as well as VLDL triglyceride (TG) and VLDL apoB kinetics, were measured with iv …
Overweight individuals with reduced insulin sensitivity often have mild to moderate hypertriglyceridemia. To investigate the role of apolipoprotein (apo)C-III metabolism in the etiology of hypertriglyceridemia in these individuals, we investigated 10 male subjects with different body weights (body mass index, 24–34 kg/m2) and insulin sensitivity (homeostasis model assessment, 4.7–35.0). Total plasma and very-low-density lipoprotein (VLDL) apoC-III kinetics, as well as VLDL triglyceride (TG) and VLDL apoB kinetics, were measured with iv injected stable isotopes. The apoC-III, TG, and apoB levels in VLDL ranged from 2.9–18.2 mg/dl, 0.49–2.89 mmol/liter, and 6.7–29.3 mg/dl, respectively. Mean production rates (PRs) were: VLDL apoC-III, 20.2 ± 4.1 μmol/d (range, 8.0–44.8); VLDL TG, 26.9 ± 4.6 mmol/d (range, 10.2–51.1); and VLDL apoB, 4.4 ± 0.8 μmol/d (range, 1.5–9.1). VLDL apoC-III PRs were significantly correlated with body mass index, homeostasis model assessment, and plasma TG (r = 0.66, P < 0.05; r = 0.80, P < 0.01; r = 0.95, P < 0.001, respectively). Similar correlations were found for plasma apoC-III PRs (r = 0.70, P < 0.05; r = 0.67, P < 0.05; r = 0.80, P < 0.01, respectively). Fractional catabolic rates (FCRs) were not significantly related to metabolic variables. VLDL TG levels were strongly related to VLDL apoC-III levels (r = 0.99, P < 0.001) and VLDL apoC-III PRs (r = 0.94, P < 0.001). VLDL apoC-III levels were more strongly correlated with VLDL TG PRs (r = 0.81, P < 0.01) than with VLDL TG FCRs or VLDL apoB FCRs (r = −0.53, P = 0.12; r = −0.37, P = 0.29). These results suggest that increased hepatic production of VLDL apoC-III is characteristic of subjects with higher body weights and lower levels of insulin sensitivity and is strongly related to the plasma concentration and level of production of VLDL TG.
Oxford University Press