[HTML][HTML] The embryotrophic activity of oviductal cell-derived complement C3b and iC3b, a novel function of complement protein in reproduction
YL Lee, KF Lee, JS Xu, QY He, JF Chiu, WM Lee… - Journal of Biological …, 2004 - ASBMB
The oviduct-derived embryotrophic factor, ETF-3, enhances the development of
trophectoderm and the hatching process of treated embryos. Monoclonal anti-ETF-3
antibody that abolishes the embryotrophic activity of ETF-3 recognized a 115-kDa protein
from the conditioned medium of immortalized human oviductal cells. Mass spectrometry
analysis showed that the protein was complement C3. Western blot analysis using an
antibody against C3 confirmed the cross-reactivities between anti-C3 antibody with ETF-3 …
trophectoderm and the hatching process of treated embryos. Monoclonal anti-ETF-3
antibody that abolishes the embryotrophic activity of ETF-3 recognized a 115-kDa protein
from the conditioned medium of immortalized human oviductal cells. Mass spectrometry
analysis showed that the protein was complement C3. Western blot analysis using an
antibody against C3 confirmed the cross-reactivities between anti-C3 antibody with ETF-3 …