Alzheimer amyloid precursor aspartyl proteinase activity in CHAPSO homogenates of Spodoptera frugiperda cells
TL Carter, G Verdile, D Groth, A Bogush… - Alzheimer Disease & …, 2004 - journals.lww.com
Presenilins are polytopic, integral proteins that control intramembranous proteolysis at the" γ-
" and" ε-" cleavage sites of the Alzheimer amyloid-β precursor protein (APP) to yield amyloid-
β peptide (Aβ) and the APP intracellular domain (AICD). We have overexpressed a
constitutively active, pathogenic form of PS1 (known as PS1 Δ exon 9) together with its
substrate, APP-C99, in Spodoperta frugiperda (Sf9) cells. Sf9 cells have been reported to
lack endogenous γ-secretase, an unexpected finding since there exists an insect …
" and" ε-" cleavage sites of the Alzheimer amyloid-β precursor protein (APP) to yield amyloid-
β peptide (Aβ) and the APP intracellular domain (AICD). We have overexpressed a
constitutively active, pathogenic form of PS1 (known as PS1 Δ exon 9) together with its
substrate, APP-C99, in Spodoperta frugiperda (Sf9) cells. Sf9 cells have been reported to
lack endogenous γ-secretase, an unexpected finding since there exists an insect …