Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
KR Tindall, TA Kunkel - Biochemistry, 1988 - ACS Publications
KR Tindall, TA Kunkel
Biochemistry, 1988•ACS PublicationsMaterials and Methods Materials. Taq DNA polymerase (lots 2A and 3) was purchased from
New England Biolabs. E. coli DNA polym-erase I, large (Klenow) fragment (Kf), was
purchased from Boehringer Mannheim Biochemicals. The AMV DNA po-lymerase was
obtained from Pharmacia. The source of all other materials has been described (Kunkel,
1984, 1985a; Kunkel et al., 1987). Methods. Preparation of M13mp2 DNA substrates,
product analysis, transfection of competentcells, plating and scoring of «-complementation …
New England Biolabs. E. coli DNA polym-erase I, large (Klenow) fragment (Kf), was
purchased from Boehringer Mannheim Biochemicals. The AMV DNA po-lymerase was
obtained from Pharmacia. The source of all other materials has been described (Kunkel,
1984, 1985a; Kunkel et al., 1987). Methods. Preparation of M13mp2 DNA substrates,
product analysis, transfection of competentcells, plating and scoring of «-complementation …
Materials and Methods
Materials. Taq DNA polymerase (lots 2A and 3) was purchased from New England Biolabs. E. coli DNA polym-erase I, large (Klenow) fragment (Kf), was purchased from Boehringer Mannheim Biochemicals. The AMV DNA po-lymerase was obtained from Pharmacia. The source of all other materials has been described (Kunkel, 1984, 1985a; Kunkel et al., 1987).
Methods. Preparation of M13mp2 DNA substrates, product analysis, transfection of competentcells, plating and scoring of «-complementation mutants, and DNA sequence analysis were performed as described (Kunkel, 1984, 1985a; Kunkel et al., 1987). DNA polymerase reactions are described in the legends to the tables.
ACS Publications