Severe liver degeneration and lack of NF-κB activation in NEMO/IKKγ-deficient mice
D Rudolph, WC Yeh, A Wakeham… - Genes & …, 2000 - genesdev.cshlp.org
D Rudolph, WC Yeh, A Wakeham, B Rudolph, D Nallainathan, J Potter, AJ Elia, TW Mak
Genes & development, 2000•genesdev.cshlp.orgPhosphorylation of IκB, an inhibitor of NF-κB, is an important step in the activation of the
transcription factor NF-κB. Phosphorylation is mediated by the IκB kinase (IKK) complex,
known to contain two catalytic subunits: IKKα and IKKβ. A novel, noncatalytic component of
this kinase complex called NEMO (N F-κB e ssential mo dulator)/IKKγ was identified
recently. We have generated NEMO/IKKγ-deficient mice by gene targeting. Mutant embryos
die at E12. 5–E13. 0 from severe liver damage due to apoptosis. NEMO/IKKγ-deficient …
transcription factor NF-κB. Phosphorylation is mediated by the IκB kinase (IKK) complex,
known to contain two catalytic subunits: IKKα and IKKβ. A novel, noncatalytic component of
this kinase complex called NEMO (N F-κB e ssential mo dulator)/IKKγ was identified
recently. We have generated NEMO/IKKγ-deficient mice by gene targeting. Mutant embryos
die at E12. 5–E13. 0 from severe liver damage due to apoptosis. NEMO/IKKγ-deficient …
Phosphorylation of IκB, an inhibitor ofNF-κB, is an important step in the activation of the transcription factor NF-κB. Phosphorylation is mediated by the IκB kinase (IKK) complex, known to contain two catalytic subunits: IKKα andIKKβ. A novel, noncatalytic component of this kinase complex called NEMO( N F-κB essentialmodulator)/IKKγ was identified recently. We have generatedNEMO/IKKγ-deficient mice by gene targeting. Mutant embryos die at E12.5–E13.0 from severe liver damage due to apoptosis.NEMO/IKKγ-deficient primary murine embryonic fibroblasts (MEFs) lack detectableNF-κB DNA-binding activity in response to TNFα, IL-1, LPS, and Poly(IC) and do not show stimulus-dependent IκB kinase activity, which correlates with a lack of phosphorylation and degradation of IκBα. Consistent with these data, mutant MEFs show increased sensitivity to TNFα-induced apoptosis. Our data provide in vivo evidence that NEMO/IKKγ is the first essential, noncatalytic component of the IKK complex.
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