[HTML][HTML] The small GTPase Rap1 is activated by turbulence and is involved in integrin αIIbβ3-mediated cell adhesion in human megakaryocytes
KMT de Bruyn, FJT Zwartkruis, J de Rooij… - Journal of Biological …, 2003 - ASBMB
The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the
control of integrin-mediated cell adhesion. Here we show that in human megakaryocytes
and several other commonly used hematopoietic cell lines such as K562, Jurkat, and THP-1,
stress induced by gentle tumbling of the samples resulted in rapid and strong activation of
Rap1. This turbulence-induced activation could not be blocked by inhibitors previously
shown to affect Rap1 activation in human platelets, such as the intracellular calcium chelator …
control of integrin-mediated cell adhesion. Here we show that in human megakaryocytes
and several other commonly used hematopoietic cell lines such as K562, Jurkat, and THP-1,
stress induced by gentle tumbling of the samples resulted in rapid and strong activation of
Rap1. This turbulence-induced activation could not be blocked by inhibitors previously
shown to affect Rap1 activation in human platelets, such as the intracellular calcium chelator …