[HTML][HTML] 2′-Fluoropyrimidine RNA-based aptamers to the 165-amino acid form of vascular endothelial growth factor (VEGF165): Inhibition of receptor binding and …

J Ruckman, LS Green, J Beeson, S Waugh… - Journal of Biological …, 1998 - ASBMB
Vascular endothelial growth factor (VEGF) has been implicated in the pathological induction
of new blood vessel growth in a variety of proliferative disorders. Using the SELEX process
(systematic evolution of ligands byexponential enrichment), we have isolated 2′-F-
pyrimidine RNA oligonucleotide ligands (aptamers) to human VEGF 165. Representative
aptamers from three distinct sequence families were truncated to the minimal sequence
capable of high affinity binding to VEGF (23–29 nucleotides) and were further modified by …