Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A
D Bevec, H Jaksche, M Oft, T Wöhl, M Himmelspach… - Science, 1996 - science.org
D Bevec, H Jaksche, M Oft, T Wöhl, M Himmelspach, A Pacher, M Schebesta, K Koettnitz…
Science, 1996•science.orgEukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor required for the function of the
human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a
set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic
copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two
nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the
HIV-1 Rev response element: Rev complex. Both mutants were constitutively expressed in …
human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a
set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic
copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two
nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the
HIV-1 Rev response element: Rev complex. Both mutants were constitutively expressed in …
Eukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor required for the function of the human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the HIV-1 Rev response element:Rev complex. Both mutants were constitutively expressed in human T cells. When these T cells were infected with replication-competent HIV-1, virus replication was inhibited. The eIF-5AM13 and eIF-5AM14 proteins blocked Rev trans-activation and Rev-mediated nuclear export.
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