Rapid, phenotypic HIV-1 drug sensitivity assay for protease and reverse transcriptase inhibitors
H Walter, B Schmidt, K Korn, AM Vandamme… - Journal of clinical …, 1999 - Elsevier
Background: Development of drug resistance is one of the major reasons for the failure of
antiretroviral therapy of HIV-1 infection. Knowing the drug sensitivity–resistance profile of
viruses present in a patient prior to treatment or change in treatment could help to optimize
therapy. Objective: Development of a rapid standardized phenotypic HIV-1 drug sensitivity
assay for protease (PR) and reverse transcriptase (RT) inhibitors. Design: The PR gene
(codons 1–99) and the 5′ part of the RT gene (codons 1–300) of HIV-1 is amplified from the …
antiretroviral therapy of HIV-1 infection. Knowing the drug sensitivity–resistance profile of
viruses present in a patient prior to treatment or change in treatment could help to optimize
therapy. Objective: Development of a rapid standardized phenotypic HIV-1 drug sensitivity
assay for protease (PR) and reverse transcriptase (RT) inhibitors. Design: The PR gene
(codons 1–99) and the 5′ part of the RT gene (codons 1–300) of HIV-1 is amplified from the …