Mucosal macrophages play a fundamental role in the regulation of immunological events
and inflammation in the small intestine. Because no information is available on normal small
intestinal macrophages, we developed a technique for the isolation and purification of
jejunal lamina propria macrophages in order to study their phenotype and activity. From
sections of normal human jejunum, lamina propria mononuclear cells were isolated by
neutral protease digestion and then subjected to counterflow centrifugal elutriation to purify …