Cloning of the human 1α, 25-dihydroxyvitamin D-3 24-hydroxylase gene promoter and identification of two vitamin D-responsive elements
KS Chen, HF DeLuca - Biochimica et Biophysica Acta (BBA)-Gene …, 1995 - Elsevier
A genomic DNA clone for 1α, 25-dihydroxyvitamin D-3 (1, 25-(OH) 2D3) 24-hydroxylase was
isolated from a human chromosome 20 library. It spans 2.42 kb, containing the first two
exons, the first and part of the second introns, and a 1.26 kb 5′-flanking region. Putative
transcription cis-elements were revealed throughout the 5′-flanking region, including TATA
box, CAAT box, GC boxes, vitamin D-responsive elements (VDRE), AP1, and AP2 sites. In a
CAT reporter gene expression assay, the 24-hydroxylase promoter with its 1.2 kb 5 …
isolated from a human chromosome 20 library. It spans 2.42 kb, containing the first two
exons, the first and part of the second introns, and a 1.26 kb 5′-flanking region. Putative
transcription cis-elements were revealed throughout the 5′-flanking region, including TATA
box, CAAT box, GC boxes, vitamin D-responsive elements (VDRE), AP1, and AP2 sites. In a
CAT reporter gene expression assay, the 24-hydroxylase promoter with its 1.2 kb 5 …