A sensitive and reproducible method for the detection of oligoclonal IgM bands
LM Villar, P González-Porqué, J Masjuán… - Journal of …, 2001 - Elsevier
We have developed a sensitive and specific isoelectrofocusing (IEF) method for the
detection of oligoclonal (OGC) IgM in the cerebrospinal fluid (CSF) and sera. In this
procedure, ampholytes, in the range pH 5–8, were used to improve the resolution, and
serum was diluted in saline to avoid IgM precipitation. Samples were treated with 50 mM
dithiothreitol (DTT) at pH 9.5 to reduce IgM and improve the migration of the protein. Using
an anti-IgM labelled with alkaline phosphatase, the limit of detection was found to be 0.1 ng …
detection of oligoclonal (OGC) IgM in the cerebrospinal fluid (CSF) and sera. In this
procedure, ampholytes, in the range pH 5–8, were used to improve the resolution, and
serum was diluted in saline to avoid IgM precipitation. Samples were treated with 50 mM
dithiothreitol (DTT) at pH 9.5 to reduce IgM and improve the migration of the protein. Using
an anti-IgM labelled with alkaline phosphatase, the limit of detection was found to be 0.1 ng …