[HTML][HTML] Nuclear import and export signals enable rapid nucleocytoplasmic shuttling of the atypical protein kinase C λ
M Perander, G Bjørkøy, T Johansen - Journal of Biological Chemistry, 2001 - ASBMB
The atypical protein kinase C (PKC) isoenzymes, λ/ι-and ζPKC, play important roles in
cellular signaling pathways regulating proliferation, differentiation, and cell survival. By
using green fluorescent protein (GFP) fusion proteins, we found that wild-type λPKC
localized predominantly to the cytoplasm, whereas both a kinase-defective mutant and an
activation loop mutant accumulated in the nucleus. We have mapped a functional nuclear
localization signal (NLS) to the N-terminal part of the zinc finger domain of λPKC …
cellular signaling pathways regulating proliferation, differentiation, and cell survival. By
using green fluorescent protein (GFP) fusion proteins, we found that wild-type λPKC
localized predominantly to the cytoplasm, whereas both a kinase-defective mutant and an
activation loop mutant accumulated in the nucleus. We have mapped a functional nuclear
localization signal (NLS) to the N-terminal part of the zinc finger domain of λPKC …