[HTML][HTML] Reversion of Ras-and phosphatidylcholine-hydrolyzing phospholipase C-mediated transformation of NIH 3T3 cells by a dominant interfering mutant of protein …
G Bjørkøy, M Perander, A Øvervatn… - Journal of Biological …, 1997 - ASBMB
The transformed phenotype of v-Ras-or Bacillus cereus phosphatidylcholine-hydrolyzing
phospholipase C (PC-PLC)-expressing NIH 3T3 cells is reverted by expressing a kinase-
defective mutant of protein kinase C λ (λPKC). We report here that extracellular signal-
regulated kinase (ERK)-1 and− 2 are constitutively activated in v-Ras-and PC-PLC-
transformed cells in the absence of added growth factors. Interestingly, the activated ERKs
were exclusively localized to the cell nucleus. Consistently, the transactivating potential of …
phospholipase C (PC-PLC)-expressing NIH 3T3 cells is reverted by expressing a kinase-
defective mutant of protein kinase C λ (λPKC). We report here that extracellular signal-
regulated kinase (ERK)-1 and− 2 are constitutively activated in v-Ras-and PC-PLC-
transformed cells in the absence of added growth factors. Interestingly, the activated ERKs
were exclusively localized to the cell nucleus. Consistently, the transactivating potential of …