Multilocus Sequence Typing of Staphylococcus aureus with DNA Array Technology
WB van Leeuwen, C Jay, S Snijders… - Journal of clinical …, 2003 - Am Soc Microbiol
WB van Leeuwen, C Jay, S Snijders, N Durin, B Lacroix, HA Verbrugh, MC Enright…
Journal of clinical microbiology, 2003•Am Soc MicrobiolABSTRACT A newly developed oligonucleotide array suited for multilocus sequence typing
(MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-
center study. MLST allele identification for the first strain collection fully agreed with
conventional strain typing. Analysis of strains from the second collection revealed that chip-
defined MLST was concordant with conventional MLST. Array-mediated MLST data were
reproducible, exchangeable, and epidemiologically concordant.
(MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-
center study. MLST allele identification for the first strain collection fully agreed with
conventional strain typing. Analysis of strains from the second collection revealed that chip-
defined MLST was concordant with conventional MLST. Array-mediated MLST data were
reproducible, exchangeable, and epidemiologically concordant.
Abstract
A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
American Society for Microbiology