A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis
N van den Braak, G Simons, R Gorkink… - Journal of …, 2004 - Elsevier
We have here applied high-throughput amplified fragment length polymorphism (htAFLP)
analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis
complex. Using 20 strains, three enzyme combinations and eight selective amplification
primer pairs, 24 AFLP reactions were performed per strain. Overall, this resulted in 480 DNA
fingerprints and more than 1200 htAFLP-amplified PCR fragments were visualised per
strain. The cumulative dendrogram correctly clustered strains from the various species …
analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis
complex. Using 20 strains, three enzyme combinations and eight selective amplification
primer pairs, 24 AFLP reactions were performed per strain. Overall, this resulted in 480 DNA
fingerprints and more than 1200 htAFLP-amplified PCR fragments were visualised per
strain. The cumulative dendrogram correctly clustered strains from the various species …