[HTML][HTML] Direct demonstration of Ca2+ binding defects in sarco-endoplasmic reticulum Ca2+ ATPase mutants overexpressed in COS-1 cells transfected with …
C Strock, M Cavagna, WE Peiffer, C Sumbilla… - Journal of Biological …, 1998 - ASBMB
Single mutations of specific amino acids within the membrane-bound region of the sarco-
endoplasmic reticulum Ca 2+(SERCA)-1 ATPase interfere with Ca 2+ inhibition of ATPase
phosphorylation by P i (1), suggesting that these residues may be involved in complexation
of two Ca 2+ that are known to bind to the enzyme. However, direct measurements of Ca 2+
binding in the absence of ATP have been limited by the low quantities of available mutant
protein. We have improved the transfection efficiency by means of recombinant adenovirus …
endoplasmic reticulum Ca 2+(SERCA)-1 ATPase interfere with Ca 2+ inhibition of ATPase
phosphorylation by P i (1), suggesting that these residues may be involved in complexation
of two Ca 2+ that are known to bind to the enzyme. However, direct measurements of Ca 2+
binding in the absence of ATP have been limited by the low quantities of available mutant
protein. We have improved the transfection efficiency by means of recombinant adenovirus …