[HTML][HTML] Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids
S An, T Bleu, W Huang, OG Hallmark, SR Coughlin… - FEBS letters, 1997 - Elsevier
S An, T Bleu, W Huang, OG Hallmark, SR Coughlin, EJ Goetzl
FEBS letters, 1997•ElsevierThe structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a
sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2
human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human
Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3
activated serum response element-driven transcriptional reporter gene in response to
sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to …
sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2
human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human
Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3
activated serum response element-driven transcriptional reporter gene in response to
sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to …
The structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2 human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3 activated serum response element-driven transcriptional reporter gene in response to sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to LPA. H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids.
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