[12] Assays of glutathione peroxidase

L Flohé, WA Günzler - Methods in enzymology, 1984 - Elsevier
Publisher Summary To determine glutathione peroxidase reliably, some factors of potential
pitfall have to be considered, for example, enzymatic side reactions of substrates (especially
when crude tissue samples are assayed), high and variable spontaneous reaction rates of
substrates, and the peculiar kinetics of the enzyme itself. With the best documented
example, the enzyme of bovine red blood cells, ping-pong kinetics with infinite limiting
maximum velocities, and Michaelis constants have been established. This means that the …