[HTML][HTML] Cell surface ceramide generation precedes and controls FcγRII clustering and phosphorylation in rafts
ABA Shakor, K Kwiatkowska, A Sobota - Journal of Biological Chemistry, 2004 - ASBMB
Despite the role of sphingolipid/cholesterol rafts as signaling platforms for Fcγ receptor II
(FcγRII), the mechanism governing translocation of an activated receptor toward the rafts is
unknown. We show that at the onset of FcγRII cross-linking acid sphingomyelinase is rapidly
activated. This enzyme is extruded from intracellular compartments to the cell surface, and
concomitantly, exofacially oriented ceramide is produced. Both non-raft and, to a lesser
extent, raft sphingomyelin pools were hydrolyzed at the onset of FcγRII cross-linking. The …
(FcγRII), the mechanism governing translocation of an activated receptor toward the rafts is
unknown. We show that at the onset of FcγRII cross-linking acid sphingomyelinase is rapidly
activated. This enzyme is extruded from intracellular compartments to the cell surface, and
concomitantly, exofacially oriented ceramide is produced. Both non-raft and, to a lesser
extent, raft sphingomyelin pools were hydrolyzed at the onset of FcγRII cross-linking. The …