[HTML][HTML] Kinase suppressor of Ras signals through Thr269 of c-Raf-1
HR Xing, R Kolesnick - Journal of Biological Chemistry, 2001 - ASBMB
We recently established a two-stage in vitro assay for KSR kinase activity in which KSR
never comes in contact with any recombinant kinase other than c-Raf-1 and defined the
epidermal growth factor (EGF) as a potent activator of KSR kinase activity (Xing, HR,
Lozano, J., and Kolesnick, R.(2000) J. Biol. Chem. 275, 17276–17280). That study, however,
did not address the mechanism of c-Raf-1 stimulation by activated KSR. Here we show that
phosphorylation of c-Raf-1 on Thr 269 by KSR is necessary for optimal activation in …
never comes in contact with any recombinant kinase other than c-Raf-1 and defined the
epidermal growth factor (EGF) as a potent activator of KSR kinase activity (Xing, HR,
Lozano, J., and Kolesnick, R.(2000) J. Biol. Chem. 275, 17276–17280). That study, however,
did not address the mechanism of c-Raf-1 stimulation by activated KSR. Here we show that
phosphorylation of c-Raf-1 on Thr 269 by KSR is necessary for optimal activation in …