Restricted use of T cell receptor V genes in murine autoimmune encephalomyelitis raises possibilities for antibody therapy
JL Urban, V Kumar, DH Kono, C Gomez, SJ Horvath… - Cell, 1988 - cell.com
JL Urban, V Kumar, DH Kono, C Gomez, SJ Horvath, J Clayton, DG Ando, EE Sercarz…
Cell, 1988•cell.comExperimental allergic encephalomyelitis(EAE) is a paralytic autoimmune disease induced in
susceptible animals by active immunization with myelin basic protein (MBP) or by passive
transfer of MBP-specific T helper (T,.,) lymphocytes. We have analyzed the T cell receptor
genes of 33 clonally distinct TH cells specific for a nonapeptide of MBP inducing EAE in BlO.
PL (H-2”) mice. All 33 TH cells used two a variable gene segments (V, 2.3, 61%; V, 4.2,
39%), the same a joining gene segment (J, 39), and two Vg and JB gene segments (VB8. 2 …
susceptible animals by active immunization with myelin basic protein (MBP) or by passive
transfer of MBP-specific T helper (T,.,) lymphocytes. We have analyzed the T cell receptor
genes of 33 clonally distinct TH cells specific for a nonapeptide of MBP inducing EAE in BlO.
PL (H-2”) mice. All 33 TH cells used two a variable gene segments (V, 2.3, 61%; V, 4.2,
39%), the same a joining gene segment (J, 39), and two Vg and JB gene segments (VB8. 2 …
Summary
Experimental allergic encephalomyelitis(EAE) is a paralytic autoimmune disease induced in susceptible animals by active immunization with myelin basic protein (MBP) or by passive transfer of MBP-specific T helper (T,.,) lymphocytes. We have analyzed the T cell receptor genes of 33 clonally distinct TH cells specific for a nonapeptide of MBP inducing EAE in BlO. PL (H-2”) mice. All 33 TH cells used two a variable gene segments (V, 2.3, 61%; V, 4.2, 39%), the same a joining gene segment (J, 39), and two Vg and JB gene segments (VB8. 2-JB2. 6, 79%; VP 13Jg 2.2, 21%). The anti-VP8 monoclonal antibody F23. 1 was found to block completely recognition of the nonapeptide by VP8 TH cells in vitro and to reduce significantly the susceptibility of BlO. PL mice to peptide-induced EAE.
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