In vivo and in vitro measurements of red cell velocity under epifluorescence microscopy

J Seki, HH Lipowsky - Microvascular research, 1989 - Elsevier
Studies of blood flow in mesentery, cremaster muscle, and small bore glass tubes were
performed to obtain a relationship between mean velocity (V mean) and red cell velocity
using the two-slit method under epifluorescence (V epi) and transillumination (V trans)
microscopy. The velocities V epi and V trans obtained in vivo for 47 measurements in
arterioles and venules (12-to 51-μm internal diameter) were linearly related by V epi= 0.83 V
trans+ 0.074, and the ratio V epi V trans decreased gradually with increasing vessel …