[HTML][HTML] Acute ablation of survivin uncovers p53-dependent mitotic checkpoint functions and control of mitochondrial apoptosis
E Beltrami, J Plescia, JC Wilkinson, CS Duckett… - Journal of Biological …, 2004 - ASBMB
Survivin is a member of the Inhibitor of Apoptosis gene family that has been implicated in
cell division and suppression of apoptosis. Here, we show that preferential ablation of the
nuclear pool of survivin by RNA interference produces a mitotic arrest followed by re-entry
into the cell cycle and polyploidy. Survivin ablation causes multiple centrosomal defects,
aberrant multipolar spindle formation, and chromatin missegregation, and these phenotypes
are exacerbated by loss of the cell cycle regulator, p21 Waf1/Cip1 in p21-/-cells. The mitotic …
cell division and suppression of apoptosis. Here, we show that preferential ablation of the
nuclear pool of survivin by RNA interference produces a mitotic arrest followed by re-entry
into the cell cycle and polyploidy. Survivin ablation causes multiple centrosomal defects,
aberrant multipolar spindle formation, and chromatin missegregation, and these phenotypes
are exacerbated by loss of the cell cycle regulator, p21 Waf1/Cip1 in p21-/-cells. The mitotic …