Analyzing mitochondrial changes during apoptosis
RA Gottlieb, DJ Granville - Methods, 2002 - Elsevier
Methods, 2002•Elsevier
Mitochondria play a central role in programmed cell death through the release of
cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize
cytochrome c translocation and loss of mitochondrial membrane potential. Flow cytometry
can also be used to measure mitochondrial membrane potential. Cytochrome c content in
cytosol and mitochondria can be determined by immunoblotting after subcellular
fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used …
cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize
cytochrome c translocation and loss of mitochondrial membrane potential. Flow cytometry
can also be used to measure mitochondrial membrane potential. Cytochrome c content in
cytosol and mitochondria can be determined by immunoblotting after subcellular
fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used …
Mitochondria play a central role in programmed cell death through the release of cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize cytochrome c translocation and loss of mitochondrial membrane potential. Flow cytometry can also be used to measure mitochondrial membrane potential. Cytochrome c content in cytosol and mitochondria can be determined by immunoblotting after subcellular fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used to study the mechanism of cytochrome c release. This article summarizes some of the more widely used methods to assess mitochondrial alterations in apoptosis.
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