The" beta-like-globin" gene domain in human erythroid cells.

D Tuan, W Solomon, Q Li… - Proceedings of the …, 1985 - National Acad Sciences
D Tuan, W Solomon, Q Li, IM London
Proceedings of the National Academy of Sciences, 1985National Acad Sciences
We have mapped the distribution of the major and minor DNase I-hypersensitive sites in the
human" beta-like-globin" gene domain. The minor DNase I-hypersensitive sites map close to
the 5'end of each of the beta-like-globin genes. Their presence is specifically associated
with the transcription of the immediate downstream beta-like-globin genes. The major
DNase I-hypersensitive sites map in what appear to be the 5'and 3'boundary areas of the
human beta-like-globin gene domain, a region estimated to span at least 90 kilobases of …
We have mapped the distribution of the major and minor DNase I-hypersensitive sites in the human "beta-like-globin" gene domain. The minor DNase I-hypersensitive sites map close to the 5' end of each of the beta-like-globin genes. Their presence is specifically associated with the transcription of the immediate downstream beta-like-globin genes. The major DNase I-hypersensitive sites map in what appear to be the 5' and 3' boundary areas of the human beta-like-globin gene domain, a region estimated to span at least 90 kilobases of DNA. These major sites are present in various erythroid cells, which express predominantly either the embryonic, the fetal, or the adult beta-like-globin genes, and seem to be involved in defining the active beta-like-globin genes domain in cells of erythroid lineage. The four major DNase I-hypersensitive sites in the 5' boundary area, when correlated with sequencing data, are shown to be located in DNA regions containing enhancer core-like sequences and alternating purine and pyrimidine bases.
National Acad Sciences