[HTML][HTML] Inhibition of phosphatidylinositol 3-kinase sensitizes vascular endothelial cells to cytokine-initiated cathepsin-dependent apoptosis
In the presence of cycloheximide, tumor necrosis factor or interleukin-1 initiates caspase
activation, loss of mitochondrial membrane potential (ΔΨ), DNA degradation, and nuclear
condensation and fragmentation characteristic of apoptotic cell death in human vascular
endothelial cells (EC). Inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002, but
not inhibition of Akt by dominant-negative mutation, also sensitizes EC to cytokine-initiated
apoptosis. Cytokine-initiated caspase activation is slower and comparatively less with …
activation, loss of mitochondrial membrane potential (ΔΨ), DNA degradation, and nuclear
condensation and fragmentation characteristic of apoptotic cell death in human vascular
endothelial cells (EC). Inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002, but
not inhibition of Akt by dominant-negative mutation, also sensitizes EC to cytokine-initiated
apoptosis. Cytokine-initiated caspase activation is slower and comparatively less with …