Differential immunogold-dextran labeling of bovine and frog rod and cone cells using monoclonal antibodies against bovine rhodopsin
D Hicks, RS Molday - Experimental eye research, 1986 - Elsevier
Eleven monoclonal antibodies against different segments of bovine rhodopsin were used
with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal
photoreceptor cells for visualization by transmission electron microscopy. Antibodies against
the C-terminus, F 1-F 2 loop and N-terminus of rhodopsin were all observed to label bovine
rod outer segments (ROS) densely, but to label rod inner segments (RIS) only sparsely. Most
antibodies bound 200–600 gold particles per μm 2 in the ROS, 10–60 gold particles per μm …
with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal
photoreceptor cells for visualization by transmission electron microscopy. Antibodies against
the C-terminus, F 1-F 2 loop and N-terminus of rhodopsin were all observed to label bovine
rod outer segments (ROS) densely, but to label rod inner segments (RIS) only sparsely. Most
antibodies bound 200–600 gold particles per μm 2 in the ROS, 10–60 gold particles per μm …