[HTML][HTML] Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin
G Bazzoni, OM Martınez-Estrada, F Orsenigo… - Journal of Biological …, 2000 - ASBMB
Junctional adhesion molecule (JAM) is an integral membrane protein that has been reported
to colocalize with the tight junction molecules occludin, ZO-1, and cingulin. However,
evidence for the association of JAM with these molecules is missing. Transfection of
Chinese hamster ovary cells with JAM (either alone or in combination with occludin) resulted
in enhanced junctional localization of both endogenous ZO-1 and cotransfected occludin.
Additionally, JAM was coprecipitated with ZO-1 in the detergent-insoluble fraction of Caco-2 …
to colocalize with the tight junction molecules occludin, ZO-1, and cingulin. However,
evidence for the association of JAM with these molecules is missing. Transfection of
Chinese hamster ovary cells with JAM (either alone or in combination with occludin) resulted
in enhanced junctional localization of both endogenous ZO-1 and cotransfected occludin.
Additionally, JAM was coprecipitated with ZO-1 in the detergent-insoluble fraction of Caco-2 …