Changes of cell volume and nitric oxide concentration in macula densa cells caused by changes in luminal NaCl concentration
R Liu, AEG Persson - Journal of the American Society of …, 2002 - journals.lww.com
The luminal NaCl concentration ([NaCl]) at the macula densa (MD) controls both
tubuloglomerular feedback (TGF) and renin release. Nitric oxide (NO) inhibits TGF sensitivity
to a great extent. The NO concentration in the MD cells is not known. This study measured
this concentration in MD cells with confocal microscopy in the isolated perfused thick
ascending limb using a NO-sensitive fluorophore 4, 5-diaminofluorescein (DAF-2). Calcein
was used to measure cell volume changes. The loop perfusion fluid was a modified Ringer …
tubuloglomerular feedback (TGF) and renin release. Nitric oxide (NO) inhibits TGF sensitivity
to a great extent. The NO concentration in the MD cells is not known. This study measured
this concentration in MD cells with confocal microscopy in the isolated perfused thick
ascending limb using a NO-sensitive fluorophore 4, 5-diaminofluorescein (DAF-2). Calcein
was used to measure cell volume changes. The loop perfusion fluid was a modified Ringer …