[HTML][HTML] Specificity of prohormone convertase 2 on proenkephalin and proenkephalin-related substrates
K Johanning, MA Juliano, L Juliano, C Lazure… - Journal of Biological …, 1998 - ASBMB
In the central and peripheral nervous systems, the neuropeptide precursor proenkephalin
must be endoproteolytically cleaved by enzymes known as prohormone convertases 1 and
2 (PC1 and PC2) to generate opioid-active enkephalins. In this study, we have investigated
the specificity of recombinant mouse PC2 for proenkephalin-related internally quenched (IQ)
peptides, for methylcoumarin amide-based fluorogenic peptides, and for recombinant rat
proenkephalin. IQ peptides exhibited specificity constants (k cat/K m) between 9.4× 10 4 m …
must be endoproteolytically cleaved by enzymes known as prohormone convertases 1 and
2 (PC1 and PC2) to generate opioid-active enkephalins. In this study, we have investigated
the specificity of recombinant mouse PC2 for proenkephalin-related internally quenched (IQ)
peptides, for methylcoumarin amide-based fluorogenic peptides, and for recombinant rat
proenkephalin. IQ peptides exhibited specificity constants (k cat/K m) between 9.4× 10 4 m …