[CITATION][C] The cytochemical localization of esterase
MS Burstone - Journal of the National Cancer Institute, 1957 - academic.oup.com
MS Burstone
Journal of the National Cancer Institute, 1957•academic.oup.comWith the LC acetate the following procedure was employed on the double-embedded
material: Twenty to 30 mg. of substrate were dissolved in 2 to 4 ml. dimethylformamide
(DMF), 5 to 10 ml. ethylene glycol mono ethyl ether (ethyl cellosolve) were added, plus 10
rul. 0.2 M buffer (tris, pH 7.1-at this pH coupling with the garnet salt produces a beautiful
violet color), and sufficient distilled water to make 50 ml. of
material: Twenty to 30 mg. of substrate were dissolved in 2 to 4 ml. dimethylformamide
(DMF), 5 to 10 ml. ethylene glycol mono ethyl ether (ethyl cellosolve) were added, plus 10
rul. 0.2 M buffer (tris, pH 7.1-at this pH coupling with the garnet salt produces a beautiful
violet color), and sufficient distilled water to make 50 ml. of
With the LC acetate the following procedure was employed on the double-embedded material: Twenty to 30 mg. of substrate were dissolved in 2 to 4 ml. dimethylformamide (DMF), 5 to 10 ml. ethylene glycol mono ethyl ether (ethyl cellosolve) were added, plus 10 rul. 0.2 M buffer (tris, pH 7.1-at this pH coupling with the garnet salt produces a beautiful violet color), and sufficient distilled water to make 50 ml. of
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