Plasma levels of apolipoprotein AI (apoA-I) are correlated with reduced incidence of heart
disease due to the critical role of this protein in reverse cholesterol transport. Because of its
diversity of function and poorly understood structure, much research has sought to
understand how the structure of apoA-I facilitates its function. A popular approach has been
the use of site-directed mutagenesis followed by structural and functional studies. There are
a wide variety of expression systems available to produce these mutant proteins including …