[HTML][HTML] Endocytosis of ligand-human parathyroid hormone receptor 1 complexes is protein kinase C-dependent and involves β-arrestin2: real-time monitoring by …
SL Ferrari, V Behar, M Chorev, M Rosenblatt… - Journal of Biological …, 1999 - ASBMB
Endocytosis and intracellular trafficking of the human parathyroid hormone receptor subtype
1 (hPTH1-Rc) and its ligands was monitored independently by real-time fluorescence
microscopy in stably transfected HEK-293 cells. Complexes of fluorescence-labeled
parathyroid hormone (PTH)-(1–34) agonist bound to the hPTH1-Rc internalized rapidly at
37° C via clathrin-coated vesicles, whereas fluorescent PTH-(7–34) antagonist-hPTH1Rc
complexes did not. A functional C terminus epitope-tagged receptor (C-Tag-hPTH1-Rc) was …
1 (hPTH1-Rc) and its ligands was monitored independently by real-time fluorescence
microscopy in stably transfected HEK-293 cells. Complexes of fluorescence-labeled
parathyroid hormone (PTH)-(1–34) agonist bound to the hPTH1-Rc internalized rapidly at
37° C via clathrin-coated vesicles, whereas fluorescent PTH-(7–34) antagonist-hPTH1Rc
complexes did not. A functional C terminus epitope-tagged receptor (C-Tag-hPTH1-Rc) was …