[HTML][HTML] Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells (∗)
CJ Sabers, MM Martin, GJ Brunn, JM Williams… - Journal of Biological …, 1995 - ASBMB
The immunosuppressive drug, rapamycin, interferes with an undefined signaling pathway
required for the progression of G 1-phase T-cells into S phase. Genetic analyses in yeast
indicate that binding of rapamycin to its intracellular receptor, FKBP12, generates a toxic
complex that inhibits cell growth in G 1 phase. These analyses implicated two related
proteins, TOR1 and TOR2, as targets of the FKBP12-rapamycin complex in yeast. In this
study, we have used a glutathione S-transferase (GST)-FKBP12-rapamycin affinity matrix to …
required for the progression of G 1-phase T-cells into S phase. Genetic analyses in yeast
indicate that binding of rapamycin to its intracellular receptor, FKBP12, generates a toxic
complex that inhibits cell growth in G 1 phase. These analyses implicated two related
proteins, TOR1 and TOR2, as targets of the FKBP12-rapamycin complex in yeast. In this
study, we have used a glutathione S-transferase (GST)-FKBP12-rapamycin affinity matrix to …